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Fibroblast growth factors and receptors are intimately connected to the extracellular matrix by their affinity to heparan sulfate proteoglycans. They mediate multiple processes during embryonic development and adult life. In this study, embryonic stem cell—derived embryoid bodies were used to model fibroblast growth factor signaling during early epithelial morphogenesis. To avoid redundancy caused by multiple receptors, we employed a dominant negative mutation of Fgfr2. Mutant-derived embryoid bodies failed to form endoderm, ectoderm, and basement membrane and did not cavitate. However, in mixed cultures they displayed complete differentiation induced by extracellular products of the normal cell. Evidence will be presented here that at least one of these products is the basement membrane or factors connected to it.
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Blowing away the fuzzy, sonic indulgences of October with propulsive, martial rhythms and shards of guitar, War bristles with anger, despair, and above all, passion. He performs the difficult task of making the universal sound personal, and the band helps him out by bringing the songs crashing home with muscular, forceful performances that reveal their varied, expressive textures upon repeated listens. U2 always aimed at greatness, but War was the first time they achieved it.